Dehydrating Delicate Specimens for Light Microscopy

Plastic embedding of transbronchial biopsy specimens for light microscopy.

Homographically generated light sheets for the microscopy of large specimens.

We compare the performance of linear and nonlinear methods for aligning the excitation and detection planes throughout volumes of large specimens in digitally scanned light sheet microscopy. An effective nonlinear method involves the registration of four corner extrema of the imaging volume via a projective transform. We show that this improves the light collection efficiency of the commonly us...

Light sheet microscopy of living or cleared specimens.

Light sheet microscopy is a versatile imaging technique with a unique combination of capabilities. It provides high imaging speed, high signal-to-noise ratio and low levels of photobleaching and phototoxic effects. These properties are crucial in a wide range of applications in the life sciences, from live imaging of fast dynamic processes in single cells to long-term observation of development...

onfocal microscopy of hick specimens

bstract. Confocal microscopy is an excellent tool to ain structural information from deep within a biological ample. The depth from which information can be exracted as well as the resolution of the detection system re limited by spherical aberrations in the laser pathway. hese spherical aberrations of the visible light can be efciently canceled by optimizing the refractive index of the mmersio...

Principles of light microscopy and its use in dermatology

.